MUC1 and MUC13 differentially regulate epithelial inflammation in response to inflammatory and infectious stimuli

YH Sheng; S Triyana; R Wang; I Das; K Gerloff; TH Florin; P Sutton; MA McGuckin

Journal article

MUC1 and MUC13 differentially regulate epithelial inflammation in response to inflammatory and infectious stimuli

YH Sheng, S Triyana, R Wang, I Das, K Gerloff, TH Florin, P Sutton, MA McGuckin

Mucosal Immunology | NATURE PUBLISHING GROUP | Published : 2013

DOI: 10.1038/mi.2012.98

Abstract

The MUC1 cell-surface mucin is highly expressed on the gastric mucosal surface, while MUC13 is highly expressed on the intestinal mucosal surface. Polymorphisms in both MUC1 and MUC13 have been linked to inflammatory bowel diseases. MUC1 can act as a decoy molecule on the apical cell surface of epithelial cells and thereby limit bacterial adherence, infection, and inflammation. In this study, we examined whether and how MUC1 and MUC13 modulate infectious and inflammatory signaling. Using gastrointestinal tissue from Muc1- or Muc13-deficient mice in ex vivo culture, MUC1 small interfering RNA (siRNA) silencing in MKN7 gastric epithelial cells, and MUC13 siRNA silencing in LS513 intestinal epi..

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University of Melbourne Researchers

Phil Sutton Author

Mike McGuckin Author

Related Projects (1)

MUCINS IN INFECTION AND INFLAMMATION

Mucosal tissues, such as the gastrointestinal and respiratory tracts, are the most common site of infectious disease. We have found that the..

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

Supported by NHMRC project grant 543704 and the Victorian Government's Operational Infrastructure Support Program. MAM and PS are supported by NHMRC Senior Research Fellowships, and THF by a NHMRC Practitioner Fellowship. We thank Hui Tong and Martina Proctor for technical assistance.